Monday, December 11, 2017

Diet-based Drosophila model of kidney stones

Chung VY, Turney BW. A Drosophila genetic model of nephrolithiasis: transcriptional changes in response to diet induced stone formation. BMC Urol. 2017 Nov 28;17(1):109. PMID: 29183349; PMCID: PMC5706311.

From the abstract: "... We have used Drosophila as a genetic model to study the transcriptional response to stone formation secondary to dietary manipulation. ... Wild-type male flies were raised on standard medium supplemented with lithogenic agents: control, sodium oxalate (NaOx) and ethylene glycol (EG). ... Crystal formation was visualized in 20%(±2.2) of flies on control diet, 73%(±3.6) on NaOx diet and 84%(±2.2) on EG diet. ... Fifty-eight genes were differentially expressed (FDR <0.05, p < 0.05) in NaOx diet and 20 genes in EG diet. ... This genetic model could be potentially used to identify the candidate genes that influence stone risk hence providing more insight to the pathogenesis of human stone disease."

No comments:

Post a Comment