Monday, July 9, 2018

Metabolic study in a Drosophila model of Huntington Disease

Sameni S, Syed A, Marsh JL, Digman MA. The phasor-FLIM fingerprints reveal shifts from OXPHOS to enhanced glycolysis in Huntington Disease. Sci Rep. 2016 Oct 7;6:34755. doi: 10.1038/srep34755. PubMed PMID: 27713486; PubMed Central PMCID: PMC5054433.

From the abstract: "Huntington disease (HD) is an autosomal neurodegenerative disorder ... Impairment in energy metabolism is a common trend in Huntington pathogenesis ... Here, we used the phasor approach and Fluorescence Lifetime Imaging Microscopy (FLIM) to measure changes between free and bound fractions of NADH as a indirect measure of metabolic alteration in living cells. Using Phasor-FLIM, pixel maps of metabolic alteration in HEK293 cell lines and in transgenic Drosophila expressing expanded and unexpanded polyQ HTT exon1 in the eye disc were developed. We found a significant shift towards increased free NADH, indicating an increased glycolytic state for cells and tissues expressing the expanded polyQ compared to unexpanded control. In the nucleus, a further lifetime shift occurs towards higher free NADH suggesting a possible synergism between metabolic dysfunction and transcriptional regulation. Our results indicate that metabolic dysfunction in HD shifts to increased glycolysis leading to oxidative stress and cell death. This powerful label free method can be used to screen native HD tissue samples and for potential drug screening."

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