Thursday, June 18, 2015

Automated fly climbing assay used to uncover loci with links to A-beta-42

Liu H, Han M, Li Q, Zhang X, Wang WA, Huang F. Automated rapid iterative negative geotaxis assay and its use in a genetic screen for modifiers of Aβ(42)-induced locomotor decline in  Drosophila. Neurosci Bull. 2015 Jun 15. PMID: 26077703.

From the abstract: "The negative-geotaxis climbing assay is used to efficiently study aging and neurodegeneration in Drosophila. To make it suitable for large-scale study, a method ... has been established by simultaneously photographing the climbing of multiple groups of flies when they are manually tapped down in test tubes. Here, we automated the assay by using a well-controlled electric motor to drive the tapping, and a homemade program to analyze the climbing height of flies. ... we examined the effect of third chromosome deficiencies on the accelerated locomotor decline in Aβ42-expressing flies, and isolated 7 suppressors and 15 enhancers."

No comments:

Post a Comment